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Applied Sciences homework help

Applied Sciences homework help. 1: You have been tasked to perform a CRISPR based knockout of your gene. Identify all candidate sgRNAs which can knockout all isoforms of your gene. Paste the excel spreadsheet with a list below.
 
 
2: Annotate in benchling where these candidate sgRNAs are
 
3: For two of the sgRNAs you have found, design PCR primers which will amplify the target site and produces a product less < 1000 bp
 
4: Indicate where in the target site the double strand break will happen.`
 
5: What is the impact on the protein coding sequence if the following NHEJ mutations occur:
(A) Single base deletion
(B) Two base deletion
(C) Three base deletion
 
 

Applied Sciences homework help

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